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10.1111/febs.16034 37 SunF.CuiL.QianJ.LiM.ChenL.ChenC.et al (2023)

[DOI] [PMC free article] [PubMed] [Google Scholar] 158.Lucena M.I., Andrade R.J., de la Cruz J.P., Rodriguez-Mendizabal M., Blanco E., Snchez de la Cuesta F

The supernatant of protein extracts was heated at 70 C for 10 min in SDS loading buffer (2% (w/v) SDS, 10% (w/v) glycerol, 0.01% (w/v) bromophenol blue and 0.125 M Tris-HCl, 0.3 M DTT, pH 6.8) and loaded on SDS-PAGE acrylamide gels for western blotting

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However, recent studies have also characterized previously unrecognized roles for tumor-draining lymph nodes (TDLNs) in cancer immunotherapy response, including acting as a reservoir for pre-exhausted CD8+ T cells and stem-like CD8+ T cells